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Honors Thesis
Title
Author
Date of Award
Spring 5-9-2020
Document Type
Honors Thesis
Department/Major
Biology
First Advisor
Dr. Jake Kerby
Second Advisor
Dr. Andrea Liebl
Third Advisor
Beate Wone
Keywords
Batrachochytrium dendrobatidis, Chytridiomycosis, Serratia marcescens, Genetic engineering
Subject Categories
Animal Diseases | Behavior and Ethology | Biodiversity | Integrative Biology | Microbiology | Other Animal Sciences
Abstract
The fungal pathogen Batrochochytrium dendrobatidis (Bd) has been threatening amphibian populations across the globe. Recent work has discovered that bacteria isolated from certain populations of amphibians protect hosts from this pathogen. This project focuses on the anti-fungal mechanisms of Serratia marcescens, a species of bacteria isolated from a Costa Rican frog that dramatically inhibited Bd in culture. Wild-type and recombinant S. marcescens was introduced to the microbiomes of a live amphibian host, Acris blanchardi (Blanchard’s Cricket Frog) and then challenged with Bd to examine the protective effects of this bacteria. The experiment includes recombinant S. marcescens with the genetic pathway responsible for the production of prodigiosin interrupted. Infranatural mac os. This work observed significantly greater movement among frogs treated with wild-type Sm when compared to the other bacteria treatments. There were also significant differences in mean mass and snout-vent length (SVL) between the washed and unwashed treatments. In particular, mean SVL was greater in washed frogs compared to unwashed frogs of the wild-type Sm and no bacteria treatments. These efforts will aid in the future conservation of amphibian species worldwide.
Recommended Citation
Mac Os Mojave
Schmidt, Emme L., 'Influences of Anti-Bd Bacteria on Amphibians and their Microbiomes' (2020). Honors Thesis. 99.
https://red.library.usd.edu/honors-thesis/99
https://red.library.usd.edu/honors-thesis/99
Included in
Animal Diseases Commons, Behavior and Ethology Commons, Biodiversity Commons, Integrative Biology Commons, Microbiology Commons, Other Animal Sciences Commons
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